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The MiQuant® SP Beads kit is a magnetic bead-based extraction kit and is intended for the isolation of nucleic acids from transduced cells for cell and gene therapy applications (e.g. CAR-T cell samples). A two-step binding mechanism allows to remove impurities in the sample matrix in combination with washing steps. Nucleic acids are eluted from the magnetic beads using an elution buffer. Extraction process can be done manually or automatically (e.g. with KingFisher™ Flex).
The MiQuant® SP Beads kit with its fast performance and high extraction efficiency is adapted to our products of the MiQuant® series, such as MiQuant® CAR-T Cell dPCR kits.
Recommended Use
For the isolation of nucleic acids from transduced cells
- CAR-T cell samples
- Viral vector samples
Kit Components
- Binding, Elution, Lysis, Rehydration and Wash Buffers
- Magnetic Bead Suspension
- Proteinase K
Package Size
Cat. No. 56-4010: 10 extractions
Cat. No. 56-4100: 100 extractions
Required Consumables
- 1-propanol, ≥ 99,5 %, p.a.
- 1.5 ml or 2 ml RNase-free reaction tubes (preferably low-bind)
Required Lab Device
- Magnetic rack
- Thermoshaker
- Vortex mixer
- Optional: Automated extraction device (e.g. KingFisher™ Flex) with corresponding DNase- and RNase-free plastic ware
Shelf Life and Storage
Shipment and storage at ambient temperature. Proteinase K has to be stored at +4 °C. The expiry date of the unopened package is marked on the package label.
