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Food Control™ qPCR is a kit for fast and reliable detection of foodborne pathogens via real-time PCR (qPCR).
Detecting bacterial contaminations is a key aspect in food safety testing that minimizes the risk of intoxicating consumers. The increasing public awareness of food safety calls for extensive microbiological quality control programs throughout the food chain production. In this context, reliability, speed, and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important.
Food Control™ qPCR is a diagnostic system for easy determination of contamination degree in agricultural or food industry via real-time PCR.
The kit enables detection of down to 10 DNA genome copies/assay of the targeted species, in about 150 minutes. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth in medium. DNA extracts can be conveniently obtained with the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user-based method.
Type of PCR
Real-time PCR. TaqMan® assay based on FAM™ and HEX™ labeled probes.
Recommended Use
Applicable in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples.
The kit is available for the detection of the following microorganisms via semi-quantitative real-time PCR:
| Microorganism | Target |
| Salmonella enterica |
invasion protein A (invA) gen |
| Yersinia enterocolitica | heat stable enterotoxin A gen |
| Shigella spp. | invasion plasmid antigen (ipaH6) gen |
| Campylobacter spp. | acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (lpxA) gen |
| Clostridium perfringens | phospholipase C alpha toxin (plc) gen |
| Shigella dysenteriae, Escherichia coli (EHEC) | Shiga Toxin 1 (stx1) gen |
| Shigella dysenteriae, Escherichia coli (EHEC) | Shiga Toxin 2 (stx2) gen |
| Escherichia coli O157 | wbdR gen |
| Escherichia coli O104 | wckD gen |
| Listeria spp. | invasion associated protein p60 (iap) gen |
| Listeria monocytogenes | listeriolysin O (hly) gen |
| Salmonella spp. | spacer-region between 16S and 23S RNA genes |
Kit Components
Lyophilized Species qPCR Mix (qPCR Mix for each species listed above): Primers / Probes / Nucleotides / Polymerase
Rehydration Buffer
Lyophilized Positive Control DNA
Lyophilized Internal Control DNA
PCR Grade Water
Package Sizes and Ordering Information
Available kits (25 reactions each) for the species listed below:
- Cat. No. 360-1025 Salmonella enterica
- Cat. No. 360-2025 Yersinia enterocolitica
- Cat. No. 360-3025 Shigella spp.
- Cat. No. 360-4025 Campylobacter spp.
- Cat. No. 360-5025 Clostridium perfringens
- Cat. No. 360-6025 Shiga Toxin 1
- Cat. No. 360-7025 Shiga Toxin 2
- Cat. No. 360-8025 Escherichia coli O157
- Cat. No. 360-9025 Escherichia coli O104
- Cat. No. 361-1025 Listeria spp.
- Cat. No. 361-2025 Listeria monocytogenes
- Cat. No. 361-3025 Salmonella spp.
Required Consumables
PCR reaction tubes, 1.5 ml reaction tubes, filter tips
Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user-based method.
Required Lab Devices
qPCR cycler with FAM™ and HEX™ filter
Pipettes
Benchtop centrifuge for 1.5 ml reaction tubes
Shelf Life and Storage
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.
Compliance
No, certification in progress.
