NAT-MAT® Cells

NAT-MAT® Cells are cryopreserved HL-60 derived macrophages, that were produced by differentiation of HL-60 myeloblasts with phorbol-12-myristate-13-acetate. They are ready-to-use and can be seeded directly into a 96-well plate. NAT-MAT® Cells can be used in combination with NAT-MAT® Extraction (Cat. No. 58-0502) and NAT-MAT® dPCR (Cat.No. 58-0503) to perform monocyte activation test (MAT) in order to detect pyrogen contamination in pharmaceuticals. NAT-MAT® Cells are not intended for cultivation. They cannot be passaged.

Components and Package Sizes

Cat. No. 58-0501: 96 reactions

NAT-MAT® Cells (Art.-Nr. 58-0501-1)

• Component: HL-60 derived macrophages
  • Quantity: 1 vial, approx. 3x10⁶ - 5x10⁶ cells/ml (exact concentration can be found in CoA)
  • Shipment: Dry ice
  • Storage conditions upon arrival: ≤-150 °C or liquid nitrogen

NAT-MAT® Accessories (Art.-Nr. 58-0501-2)

• Component #1: 96-well Zellkulturplatte
  • Quantity: 1 plate
  • Shipment: Ambient temperature
  • Storage conditions upon arrival: Umgebungstemperatur
• Component #2: Cell Medium
  • Quantity: 1 bottle 30 ml
  • Shipment: Ambient temperature
  • Storage conditions upon arrival: +2 bis +8 °C
• Component #3: Assay medium
  • Quantity: 1 bottle 20 ml
  • Shipment: Ambient temperature
  • Storage conditions upon arrival: +2 bis +8 °C

Please note, that NAT-MAT® Cells components are shipped in two separate packages due to different shipment conditions.

Required Lab Device and Consumables

• Liquid nitrogen or ≤-150 °C ultra-freezer
• CO² Incubator
• Laminar airflow cabinet
• Water bath
• Centrifuge (for 15 ml falcons)
• 15 ml falcon tubes
• Fetal bovine serum
• Inverted microscope (optional)
• Reference standard endotoxin (RSE), e.g. preparations from USP or EP (BRP)
• Sterile, non-pyrogenic water (LAL water)
• 2 relevant non-endotoxin pyrogens (optional)
• Pyrogen-free glass tubes
• Microcentrifuge
• Vortex mixer
• Pipettes with corresponding filter tips (10 μl, 100 μl, and 1000 μl)
• Multi-channel pipette (optional)

Shipment, Shelf Life, and Storage

Shipment with dry ice and ambient temperature. The expiry date of the unopened package is marked on the package label. The cells must be stored at ≤-150 °C or liquid nitrogen until use. The cell culture media must be stored at +2 to +8°C until use.

Do you prefer an all-in-one system, that provides all necessary components for performing MAT?
Have a look at NAT-MAT® All-in-One System that consists of NAT-MAT® Cells, NAT-MAT® Extraction and NAT-MAT® dPCR.