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NAT-MAT® dPCR provides a lyophilized primer/probe mix and parallelly measures the gene expression of two cytokines – IL-1β (HEX™) and TNF-α (Cy5®) – and one housekeeping gene (FAM™) in a triplex PCR reaction. NAT-MAT® dPCR can be used in combination with NAT-MAT® Cells (Cat. No. 58-0501) and NAT-MAT® Extraction (Cat. No. 58-0502) to perform monocyte activation test (MAT) in order to detect pyrogen contaminations in pharmaceuticals.
Type of PCR
Digital PCR (dPCR)
Components
- NAT-MAT® Mix (lyophilized)
- Positive Control DNA (lyophilized)
- dPCR grade Water
Package Size
Cat. No. 58-0503: 96 reactions
Result Evaluation
Absolute Quantification
Required Lab Device and Consumables
- QIAcuity® digital PCR system
- QIAcuity® Nanoplate 8.5k 96-well
- QIAcuity® OneStep Advanced Probe Kit
- Nanoplate tray
- Nanoplate seal
- 1.5 ml reaction tubes
- Microcentrifuge for 1.5 ml PCR tubes
- Vortex mixer
- Pipettes with corresponding filter tips
- Pipetting robot (optional, e.g. QIAgility® with nanoplate adapter)
Shipment, Shelf Life, and Storage
Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.
Do you prefer an all-in-one system, that provides all necessary components for performing MAT?
Have a look at NAT-MAT® All-in-One System that consists of NAT-MAT® Cells, NAT-MAT® Extraction and NAT-MAT® dPCR.
