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NAT-MAT® Extraction is a magnetic bead-based extraction kit and was specifically developed for nucleic acid extraction from NAT-MAT® Cells. Extraction process can be performed automatically (e.g. with KingFisher™ Flex). Manual extraction is also possible but not recommended.
NAT-MAT® Extraction can be used in combination with NAT-MAT® Cells (Cat. No. 58-0501) and NAT-MAT® dPCR (Cat. No. 58-0503) to perform monocyte activation test (MAT) in order to detect pyrogen contaminations in pharmaceuticals.
Components
- Binding, Elution, Lysis, Rehydration, and Wash Buffers
- Magnetic Bead Suspension
- Proteinase K
Package Size
Cat. No. 58-0502: 96 reactions
Required Lab Device and Consumables
- 1-propanol, ≥99,5 %, p.a.
- Automated extraction device (e.g. KingFisher™ Flex)
- 96-well plates for automated extraction device
- For manual extraction (not recommended): thermal shaker and magnetic rack
Shipment, Shelf Life, and Storage
Shipment and storage at ambient temperature. The expiry date of the unopened package is marked on the package label. Proteinase K has to be stored at +4 °C upon arrival.
Do you prefer an all-in-one system, that provides all necessary components for performing MAT?
Have a look at NAT-MAT® All-in-One System that consists of NAT-MAT® Cells, NAT-MAT® Extraction and NAT-MAT® dPCR.
