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dPCR Validation Standard HEK-293
Quantified DNA standard derived from genomic HEK-293 DNA to support dPCR-based residual DNA analysis.
- Quantified HEK-293 genomic DNA
- Suitbale for generating standard curves via serial dilution
- For determining a matrix-specific conversion factor that converts [copies/µl] into [pg/µl] to provide quantification of residual DNA in conformity with regulatory guidelines
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Product Description
The dPCR Validation Standard HEK-293 contains pure, quantified genomic DNA from HEK-293 cell line. This product is designed as an aid for the dPCR-based analysis of residual DNA.
Recommended Use
The dPCR Validation Standard HEK-293 can be used to generate a standard curve for the HEK-293 cell line by serial dilution. With this standard curve, the measured DNA in copies/µl can be converted to fg/µl to obtain a quantification of residual DNA in accordance with regulatory guidelines. In addition, the dPCR Validation Standard can be used in downstream processes to check the residual DNA concentration of the processed sample during different purification processes.
Content
Each dPCR Validation Standard contains 1 tube with 1 ng of lyophilised genomic DNA and 1 tube with rehydration buffer.
Available Species and Ordering Information
Cat. No. 58-0221: HEK-293
Result Evaluation
This product is designed as an aid for dPCR-based residual DNA analysis:
• MiQuant® Residual DNA dPCR HEK-293
Required Lab Device and Consumables
• 1.5 ml reaction tubes (low-bind, DNA- and RNA-free)
• Microcentrifuge for 1.5 ml PCR tubes
• Vortex mixer
• Pipettes with corresponding filter tips
Shelf Life and Storage
Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month. Repeated freezing and thawing must be avoided.
