The dPCR Validation Standards contain pure, quantified genomic DNA of the selected species or cell lines. These products are designed as a tool to assist Residual DNA dPCR-based analysis.
Each dPCR Validation Standard can be applied to generate genomic DNA Standard curves for the specific species or cell lines of interest, by serial dilution. A standard curve can be used to convert the measured DNA in copies/µl through dPCR in fg/µl to provide a quantification of the residual DNA in conformity to regulatory guidelines. In addition, the dPCR Validation Standards can be used during downstream processes to verify the residual DNA concentration of the processed sample during several purification processes.
Each dPCR Validation Standard contains 1 vial containing 1 ng of lyophilized genomic DNA and 1 vial of Rehydration Buffer. The expiry date of the unopened package is given on the package label.
Available Species and Ordering Information
Cat. No. 58-0201:E. coli
Cat. No. 58-0211:CHO
Cat. No. 58-0221:HEK-293
These products are designed as a tool to assist Residual DNA dPCR-based analysis:
• Pipettes with corresponding filter tips (10 and 100 μl)
• Microcentrifuge for 1.5 ml reaction tubes
Shelf Life and Storage
The components must be stored at +2 °C to +8 °C until use. Once rehydrated, the dPCR Validation Standards can be stored at ≤ -18 °C for a maximum of 30 days. Repeated freeze-thaw cycles must be avoided.