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The MiQuant® Residual DNA dPCR HEK-293 is designed for the quantitative detection of residual DNA from HEK-293 cells in chemical and biological matrices.
The MiQuant® Residual DNA dPCR HEK-293 is designed for use with the QIAGEN® QIAcuity® digital PCR (dPCR) system as a novel rapid, robust and sensitive method for the detection of residual host cell DNA.
Type of PCR
Digital PCR (dPCR)
Recommended Use
To test product quality, the amount of residual DNA in a drug's final dosage form must follow guidelines established by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), the European Pharmacopoeia, and other regulatory agencies.
Kit Components
- dPCR HEK-293 Mix in aliquots of 24 reactions each
- Rehydration Buffer
- Positive Control DNA
- Internal Control DNA
- dPCR Grade Water
Package Size
Cat. No. 58-0121: 24 reactions
Cat. No. 58-0122: 4 x 24 reactions
Result Evaluation
Absolute Quantification
Required Consumables
- PCR reaction tubes
- PCR Clean™ (15-2025, 15-2001)
- PCR reaction tubes or plates
- MiQuant® dPCR Validation Standard HEK-293 (Cat. No. 58-0221)
Required Lab Device
- QIAcuity® digital PCR cycle
- QIAcuity® 24Well 26K Nanoplates
- Microcentrifuge for PCR reaction tubes
- Pipetting equipment
Shelf Life and Storage
The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be store at ≤ -18°C for a maximum of one month.
