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MiQuant® CAR-T Cell Lenti – dPCR
Intended for the determination of vector copy numbers (VCN) in transduced cells.
- In-process control and release testing
- Compatible with different dPCR/ddPCR™ platforms
- Quantification of VCN without standard curve
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Validation Report
Validation Report for MiQuant® CAR-T Cell Lenti – dPCR
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Product Description
The MiQuant® CAR-T Cell Lenti – dPCR kit is intended for the determination of vector copy numbers (VCN) in transduced cells to determine the average copies of integrated lentiviral vectors per cell genome after ex vivo transduction via digital PCR (dPCR). This kit provides a specific primer/probe mix and offers an open-platform solution for use with various dPCR devices (QIAcuity®, ddPCR™, Quantstudio™ Absolute Q™). MiQuant® CAR-T Cell Lenti – dPCR works for all HIV-1 based lentivirus transduction systems.
Type of PCR
Digital PCR (dPCR)
Recommended Use
MiQuant® CAR-T Cell Lenti – dPCR can be used for the characterization of cellular products and for in-process control as well as final QC release testing in cell therapy production processes.
Besides these applications the MiQuant® CAR-T Cell Lenti – dPCR kit can also be used for determining VCN in cell culture samples in research and development and is applicable for human or other mammalian cells (such as mice and rats).
Kit Components
- MiQuant® Lenti Mix (lyophilized)
- Positive Control DNA (lyophilized)
- dPCR grade Water
Package Size
Cat. No. 58-0701: 35 µl primer/probe mix
Cat. No. 58-0702: 140 µl primer/probe mix
Since every dPCR device requires different PCR reaction volumes, the number of PCR reactions are shown in the following table.
Result Evaluation
Absolute Quantification
Required Lab Device and Consumables
- Digital PCR device with corresponding consumables (plates or stripes)
- QIAcuity®
- Quantstudio™ Absolute Q™
- QX600™ or QX200™ ddPCR™
- Corresponding Mastermix (QIAcuity® Probe PCR Kit (4x), Absolute Q™ DNA Digital PCR Master Mix (5x), ddPCR™ Multiplex Supermix (4x) or ddPCR™ Supermix for Probes (2x))
- MiQuant® SP Beads (sample preparation kit, Cat. No. 56-4010/-4100)
- 1.5 ml reaction tubes (low-bind, DNA- and RNA-free)
- Microcentrifuge for 1.5 ml PCR tubes
- Vortex mixer
- Pipettes with corresponding filter tips
Optional:
- Restriction enzymes (EcoRI-HF and NruI-HF; 20 U/μl)
- PCR Clean™ (Cat. No. 15-2025 and 15-2001)
Shelf Life and Storage
Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.
Calculate the Vector Copy Number with our tool.
Data Analysis Application
Minerva Biolabs offers a free sample of this product for testing:
Request free sample
