AAV Vector Titer Determination
Determination of adeno-associated virus vector titer by digital PCR with MiQuant® AAV.
- Fast and easy workflow
- Quantification independent of AAV serotype
- TruTiter™ technology allows highly accurate results by only amplifying DNA in intact capsids
- No DNA extraction or standard curve required
- Suitable for QIAcuity®, ddPCR™ and Quantstudio™ Absolute™ Q
- In-Process Control and Release Testing
- Lyophilized kit components simplify logistics and storage
Gene therapy represents one of the most ground-breaking advancements in modern medicine that has rapidly evolved over the last years leading to improved methods of ap-plications. Adeno-associated virus (AAV) vectors are among the most prominent viral vectors for in vivo gene therapy that target the healthy gene material to the correct location in the organism which enables the mutated gene to be exchanged by the healthy one.
Viral genome titer is a critical quality attribute in AAV characterization for safe and efficacious gene therapy products. Specificity of AAV vectors as well as the concentration of the vector titer must be determined throughout the production process and for final release testing.
The MiQuant® AAV kits have been developed for the determination of AAV vector genome titer. Different kit versions enable the specific detection of AAV ITR 2/6 (including ITR 1, 3 & 7) or ITR 5.
TruTiter™ technology - powered by Promega
The integrated TruTiter™ technology makes it possible to amplify only DNA of intact capsids, while free DNA and DNA from broken capsids is masked and cannot be amplified. This allows highly accurate measurement of AAV genome titer an eliminates the need for a DNase treatment step.
In contrast to TruTiter™, DNase cannot access the not yet released DNA of broken capsids, which makes the TruTiter™ more accurate.
Application Areas
For in-process control and final QC release testing.
Find more information on Minerva Biolabs' MiQuant® AAV in our flyer.
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Testers wanted!
Minerva Biolabs is looking for suitable testers for MiQuant® AAV.
Please contact us at miquant@minerva-biolabs.com to get further information.