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PCR-based detection methods for standard applications as well as for microbial contamination detection require specific DNA standards for quantification, calibration and assessment of the method specificity and sensitivity. Standardization and quantification of nucleic acid detection is a difficult task due to the lack of reliable standards for low nucleic acid copy numbers. When extracted from plant and animal tissues, food matrices and various other solutions, DNA standards might often contain PCR-inhibiting substances which in turn lead to underestimation of the target nucleic acid amount or to false negative results.
Minerva Biolabs´ calibration reagents (PCR Quantification Standards) contain genomic DNA extracted from defined microorganisms at low culture passage, partially sequenced (quality control) and titrated to obtain a defined number of genome copies to generate standard curves. The number of genomic copies to create standard curves was determined by quantitative PCR.
Our DNA standards for specificity testing of newly developed assays (Genomic DNA Extracts) contain genomic DNA extracted from a variety of microorganisms at low culture passage.
Minerva Biolabs´ 10CFU™ and 100CFU™ (new!) Mycoplasma Sensitivity Standards are intended for the validation of the robustness and sensitivity of nucleic acid-based mycoplasma contamination tests according to European Pharmacopoeia (EP, Chapter 2.6.7) and Japanese Pharmacopoeia (JP, Chapter G3). Besides being used in combination with our detection kits, you can also use our 10CFU™ and 100CFU™ to validate your own PCR systems or third-party assays.