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The AquaScreen® Legionella species qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Legionella in water samples. The supplied primer set is specific for a broad range of legionella species, but does not detect other waterborne bacteria as required by ISO/TS 12869.
Type of PCR
Quantitative real-time PCR (qPCR)
Recommended Use
Applicable for water testing as described in ISO/TS 12869, in research and industry for QA testing of process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.
Kit Components
Lyophilized PCR mix: Primers / Probes / Nucleotides / Polymerase in aliquots of 25 reactions each
Rehydration Buffer
Lyophilized Positive Control DNA
Lyophilized Internal Control DNA
PCR Grade Water
Package Sizes
- Cat. No. 33-2025 25 reactions
- Cat. No. 33-2100 100 reactions
- Cat. No. 33-2250 250 reactions
Required Consumables
PCR reaction tubes and filter tips
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).
Required Lab Devices
qPCR cycler with filter sets for FAM™ and ROX™
Pipettes
Shelf Life and Storage
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.
Compliance
AFNOR XP T90-471 and ISO/TS 12869
