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PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks. The kit can detect more than 45 bacteria genera, based on alignment analysis.
Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application, specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Bacteria Mix: Primers / Nucleotides / Internal Control DNA / Polymerase in aliquots of 25 reactions each (lyophilised)
Rehydration Buffer
Positive Control DNA (lyophilised)
PCR-grade Water
Gel electrophoresis at endpoint of PCR
PCR reaction tubes and filter tips
PCR cycler
Agarose gel electrophoresis and DNA stain
Pipetting equipment
Microcentrifuge
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.
Fig. Amplified PCR products are visualized by standard gel electrophoresis. Amplification of the Internal Control DNA (low molecular weight band) indicates a successful PCR and the lack of PCR-inhibiting components in the sample. A band in correspondence of the target (ca. 460 bp) implies the presence of a bacterial contamination.
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