- Online only

Let us notify you about our product updates.
Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR. All Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) species so far described as contaminants of cell cultures and media components are specifically detected by amplification of a highly conserved rRNA operon, or more specifically, of the 16S rRNA coding region in the mycoplasma genome. The mycoplasma-specific amplification is detected at 265-278 bp. False negative results due to PCR inhibitors or improper DNA extraction can be ruled out by using the provided internal amplification control, which is detected at 191 bp.
Conventional, endpoint PCR
Applicable in research and industry for the direct detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) contaminations in cell cultures and biopharmaceuticals. Approved for testing according to EP 2.6.7 and JP G3. Intended for research use only. Not recommended for clinical diagnostics.
Gel electrophoresis at endpoint of PCR
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.
Use of Venor®GeM Classic for QA testing of biologicals like master and working cell banks, autologous cells, culture media, bulk harvest and final product testing according to EP 2.6.7 and JP G3 is applicable after appropriate sample preparation and process validation.
Fig. Amplified PCR products are visualized by standard gel electrophoresis.