Venor®GeM Advance

Venor®GeM Advance is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.

Type of PCR

For conventional, endpoint PCR with gel electrophoresis

Recommended Use

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Pre-dispensed and lyophilized Primer sets/ Nucleotides / Polymerase and Internal Control DNA, in 0.2 ml PCR reaction tubes
Rehydration buffer including gel loading buffer and running dye
Lyophilized Positive Control DNA pre-dispensed in 0.2 ml PCR reaction tubes

Package Sizes

• Cat. No. 11-7024 24 Reactions
• Cat. No. 11-7048 48 Reactions
• Cat. No. 11-7096 96 Reactions
• Cat. No. 11-7240 240 Reactions

Result Evaluation

Gel electrophoresis at endpoint of PCR

Required Consumables

Agarose and DNA-specific dye for gel staining

Required Lab Devices

PCR cycler for conventional PCR
Agarose gel electrophoresis and gel documentation system
Pipetting equipment
Tube centrifuge

Shelf Life and Storage

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label.
At the time of the experiments, the rehydrated reagents should be used within 1 hour.

EP 2.6.7 compliance

No

Fig. Amplified PCR products are visualized by standard gel electrophoresis.

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