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Ready-to-use conventional PCR assay for fast mycoplasma detection in typical research applications.
- Simple workflow with minimal hands-on time due to pre-aliquoted PCR strips
- easy results interpretation
- Reliable detection of >90 mycoplasma species
Technical Note for Venor®GeM Advance
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Please provide your first name. Please provide your last name. The e-mail seems to be missing or incorrect. Please provide your company/institution. Send Technical NoteVenor®GeM Advance is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.
For conventional, endpoint PCR with gel electrophoresis
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Pre-dispensed and lyophilized Primer sets/ Nucleotides / Polymerase and Internal Control DNA, in 0.2 ml PCR reaction tubes
Rehydration buffer including gel loading buffer and running dye
Lyophilized Positive Control DNA pre-dispensed in 0.2 ml PCR reaction tubes
Gel electrophoresis at endpoint of PCR
Agarose and DNA-specific dye for gel staining
PCR cycler for conventional PCR
Agarose gel electrophoresis and gel documentation system
Pipetting equipment
Tube centrifuge
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label.
At the time of the experiments, the rehydrated reagents should be used within 1 hour.
No

Fig. Amplified PCR products are visualized by standard gel electrophoresis.
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