Venor®GeM Advance is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.
Type of PCR
For conventional, endpoint PCR with gel electrophoresis
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Pre-dispensed and lyophilized Primer sets/ Nucleotides / Polymerase and Internal Control DNA, in 0.2 ml PCR reaction tubes Rehydration buffer including gel loading buffer and running dye Lyophilized Positive Control DNA pre-dispensed in 0.2 ml PCR reaction tubes
Cat. No. 11-7024 24 Reactions
Cat. No. 11-7048 48 Reactions
Cat. No. 11-7096 96 Reactions
Cat. No. 11-7240 240 Reactions
Gel electrophoresis at endpoint of PCR
Agarose and DNA-specific dye for gel staining
Required Lab Devices
PCR cycler for conventional PCR Agarose gel electrophoresis and gel documentation system Pipetting equipment Tube centrifuge
Shelf Life and Storage
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. At the time of the experiments, the rehydrated reagents should be used within 1 hour.
EP 2.6.7 compliance
Fig. Amplified PCR products are visualized by standard gel electrophoresis.