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Mynox® Gold
Combined reagent for gentle mycoplasma elimination, ideal for sensitive cell lines.
- Gentle but efficient mycoplasma removal for sensitive cell cultures
- Biophysical mode of action through surfactin
- With selected antibiotics
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Technical Notes
Technical Note for Mynox® Gold
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Product Description
Mynox® Gold is a further development of the classic Mynox®. Mynox® Gold is a combination of a standard antibiotic ciprofloxacin with the biological Mynox® reagent surfactin. One application includes an initial treatment that eliminates most mycoplasmas without affecting the cells. The follow-up treatments destroy the remaining mycoplasmas. The mode of action of Mynox® Gold is based on a biophysical mechanism to integrate into the membrane of mycoplasmas and thereby impair their integrity.
Benefits
- Functionality test
Technical Note on the functional testing of Mynox® Gold. Removal of mycoplasma from a Vero cell line contaminated with mycoplasma.
- Low resistance risk
The combination of the standard antibiotic ciprofloxacin and the Mynox® reagent surfactin virtually eliminates the possibility of resistance to Mynox® Gold when eliminating mycoplasmas.
- Highly effective
The eradication efficiency is close to 100 %.
- Mycoplasma removal from virus stocks
Protocol for the pre-treatment of virus stocks available as a Technical Note.
Recommended Use
Mynox® Gold is intended for research use only and not for clinical applications. Not applicable for the direct treatment of cells that serve as host cells for Chlamydia or other bacterial host cells. The level of antibiotics in the product may affect the integrity of the parasites.
Content
One application of Mynox® Gold contains one vial for the starter treatment and three vials for the main treatment. Each vial contains 500 µl as a sterile, ready-to-use solution.
Package Sizes
A single application is sufficient to permanently remove mycoplasma from a cell culture.
- Cat. No. 10-0201 2 applications
- Cat. No. 10-0501 5 applications
- Cat. No. 10-1001 10 applications
Result Evaluation
Elimination success should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs' Venor®GeM Mycoplasma PCR Detection Kits.
Required Consumables
25 cm2 cell culture flasks or tissue culture petri dishes (60 mm Ø)
Standard cell culture plasticware
Required Lab Devices
Standard cell culture equipment (incubator, centrifuge, pipettes, etc.)
Shelf Life and Storage
Store the components at +2 °C to +8 °C until the expiry date indicated on the label. Protect from light.
