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Mynox®
Non-antibiotic reagent for eliminating mycoplasma from contaminated cell cultures.
- Suitable for robust cell lines and situations requiring a stronger treatment
- Biophysical mode of action through surfactin
- Without antibiotics
- Removes mycoplasma within a few days
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Technical Notes
Technical Note for Mynox®
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Product Description
Mynox® is a mycoplasma removal agent for treatment of contaminated cell cultures. Mycoplasma elimination is achieved in only 6 - 8 days (plus 4 passages for DNA washout).
The classic Mynox® avoids the use of typical antibiotics and instead contains surfactin, a cyclic lipopeptide. This prevents the development of resistant strains. The mode of action of Mynox® is based on a biophysical mechanism to integrate into the membrane of mycoplasmas and thereby impair their integrity
Recommended Use
Mynox® is intended for research use only. Not applicable for clinical treatment. Protocols for the direct treatment of viruses are described in the product manual.
Content
Mynox® is a sterile, ready-to-use solution, aliquoted per tube for single use, 200 µl per vial.
Package Sizes
1 application can be used to permanently eliminate mycoplasma from one cell culture.
- Cat. No. 10-0200 2 applications
- Cat. No. 10-0500 5 applications
- Cat. No. 10-1000 10 applications
Result Evaluation
Successful elimination of mycoplasma should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs’s Venor®GeM Mycoplasma PCR Detection Kits.
Required Consumables
Tissue culture petri dish (60 mm Ø)
Standard cell culture plasticware
Required Lab Devices
Standard cell culture equipment (incubator, centrifuge, pipettes, etc.)
Shelf Life and Storage
Store the components at +2 °C to +8 °C until the expiry date indicated on the label.
Fig. Electron micrograph showing the effects of Mynox® when applied to contaminated cell cultures. (A) Mycoplasma contaminated cell culture before treatment. (B) With one application of Mynox® membranes of all mycoplasma are destroyed while the tissue cell membrane is unaffected. Black arrows indicate mycoplasmas, white arrows indicate parts of a tissue cell.
