Venor®GeM OneStep is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be dispensed into the reaction tubes according to the sample number. Only samples or controls need to be added prior to PCR.
Type of PCR
For conventional, endpoint PCR with gel electrophoresis
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Lyophilized Mix: Primer sets / Nucleotides / Internal Control DNA and polymerase at optimized concentrations in aliquots of 25 reactions each Rehydration Buffer Lyophilized Positive Control DNA PCR Grade Water
Cat. No. 11-8025 25 Reactions
Cat. No. 11-8050 50 Reactions
Cat. No. 11-8100 100 Reactions
Cat. No. 11-8250 250 Reactions
Gel electrophoresis at endpoint of PCR
Agarose gels and DNA stains PCR reaction tubes and pipette filter tips
Required Lab Devices
PCR cycler for conventional PCR Gel electrophoresis and documentation system Pipetting equipment Microcentrifuge
Shelf Life and Storage
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.