Venor®GeM qEP utilises quantitative, real-time PCR (qPCR) for fast (ca. 3 h) and reliable screening of cell culture supernatants for mycoplasma contaminations. The kit can be used in combination with any type of real-time PCR cycler able to detect the fluorescence dyes FAM™ and HEX™. All Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) species so far described as contaminants of cell cultures and media components are specifically detected by amplifying a highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. The mycoplasma-specific amplification is detected at 520 nm (FAM™ channel). False negative results due to PCR inhibitors or improper DNA extraction can be ruled out by using the provided internal amplification control, which is detected at 560 nm (HEX™ channel).
Type of PCR
TaqMan® based qPCR Assay with FAM™ and HEX™ labeled probes
Applicable in research and industry for the direct detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) contaminations in cell cultures and biopharmaceuticals. For testing according to EP 2.6.7, USP <1071> and JP G3. Notice! Not applicable for the ABI 7500/7500 Fast in combination with the AccuSEQ software!
Lyophilized Mix: Primer sets / Nucleotides/ Probes / Polymerase in aliquots of 25 reactions each Rehydration Buffer Lyophilized Internal Control DNA Lyophilized Positive Control PCR Grade Water
Cat. No. 11-9025 25 Reactions
Cat. No. 11-9100 100 Reactions
Cat. No. 11-9250 250 Reactions
Cycler-based, real-time PCR
PCR reaction tubes Optional for process validation and EP 2.6.7 / JP G3 compliant testing: Internal Control DNA Extra (4 vials for 300 µl each of internal amplification control; Cat. No. 11-9905) 10CFU™ Sensitivity Standards available for all EP 2.6.7 / JP G3 listed Mycoplasma species
Required Lab Devices
qPCR cycler with FAM™ and HEX™ filter Pipetting equipment Benchtop centrifuge for 1.5 ml reaction tubes
Shelf Life and Storage
Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.
EP 2.6.7 / USP <1071> / JP G3 Compliance
Please note that validation data are provided for information purpose only. EP 2.6.7 clearly states “Where commercial kits are used …, documented validation points already covered by the kit manufacturer can replace validation by the user. Nevertheless, the performance of the kit with respect to its intended use has to be demonstrated by the user (e.g. detection limit, robustness, cross-detection of other classes of bacteria.”. Please feel free to contact us if you need further assistance.
Fig. Amplified dilution series of Mycoplasma fermentans, performed on a Corbett RotorGene®6000.