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Proteinase K is a proteolytic enzyme. The enzyme uses Ca2+ ions for structural stability. Due to the high total activity of the enzyme, lysis can be carried out in the presence of EDTA. Proteinase K is therefore excellently useful to digest contaminating proteins and improve the extraction of nucleic acids from various biological samples.
The Venor®GeM Sample Preparation Kit from Minerva Biolabs is designed for highly efficient extraction of mycoplasma DNA from standard samples, like cell culture supernatants containing up to 106 cells. However, other samples matrices like cell pellets, serum, or fermentation products may contain proteins at a concentration higher than 10 mg/ml. These samples may require an additional treatment with Proteinase K to increase the overall DNA yield and reach highest sensitivity.
Optional in combination with Venor®GeM Sample Preparation Kit (Minerva Biolabs) or Microsart® AMP Extraction Kit (Sartorius):
Use 10 µl of the rehydrated Proteinase K to treat each sample immediately after addition of Conditioner (Venor®GeM Sample Preparation Kit) or Buffer A (Microsart® AMP Extraction). Continue as indicated in the respective manuals.
Proteinase K: 1 vial, lyophilized
Rehydration Buffer: 1 vial
Dissolve the lyophilized Proteinase K in 550 µl of the included rehydration buffer.
- Cat. No. 56-0002 50 extractions
Shelf Life and Storage
The lyophilized product shall be stored at +2 to +8 °C. The rehydrated stock solution must be stored at ≤ -18 °C. Avoid repeated freezing and thawing. Preparation of aliquots might be required if more than 5 freezing/thawing cycles are necessary.