Mynox® Gold

Cat. No.: 10-0201


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Mynox® Gold Technical Note for Mynox® Gold

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Mynox® Gold is a further developed product formulation of the classic Mynox® reagent that combines a standard antibiotic with the Mynox® ingredients. One application consists of 4 vials: a starter and 3 main treatments. The starter treatment kills most of the mycoplasma particles without harming the cells, whereas the main treatment leads to a permanent eradication of the remaining mycoplasmas.


  • No cytotoxicity

    Cytotoxicity is at undetectable levels, with no changes in morphology during treatment for most cell types.

  • Low resistance risk

    Due to the combination of an antibiotic and a reagent showing a biophysical mode of action directly eliminating the mycoplasma, formation of resistances against Mynox® Gold is most unlikely.

  • Highly effective

    The eradication efficiency is close to 100 %.

Recommended Use

Mynox® Gold is intended for research use only. Not applicable for clinical treatment. Not applicable for the direct treatment of cells harboring Chlamydia or other bacteria as host systems. The antibiotic component of the product might affect the integrated microorganisms. Protocols for the direct treatment of viruses are available upon request.


1 application of Mynox® Gold contains 1 vial for the starter treatment and 3 vials for the main treatment. Each vial contains 500 µl as a sterile, ready-to-use solution.

Package Sizes

1 application can be used to cure one cell culture permanently from mycoplasma burden.

  • Cat. No. 10-0201 2 applications
  • Cat. No. 10-0501 5 applications
  • Cat. No. 10-1001 10 applications

Result Evaluation

Elimination success should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs’s Venor®GeM Mycoplasma PCR Detection Kits.

Required Consumables

25 cm2 cell culture flasks or tissue culture petri dishes (60 mm Ø)
Standard cell culture plasticware

Required Lab Devices

Standard cell culture equipment (incubator, centrifuge, pipettes, etc.)

Shelf Life and Storage

Store the components at +2 °C to +8 °C until the expiry date indicated on the label. Protect from light.


Fig. Cytotoxicity of Mynox® Gold on contaminated cells. Adherent cell lines (Vero, ML/S MDBK and RK13) were treated with Mynox® Gold according to the protocol 4 days of incubation. The cultures were inactivated and stained as described by Flick & Gifford. Untreated cultures were used as reference (100 %).

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